Sentence examples for dye spot from inspiring English sources

This improvement in performance is further reflected by the smaller sum of absolute errors across dye spot locations for the SPLASSO, 8.6 as compared to the the LASSO, 16.

The spectral signatures presented in Fig. 2 were estimated from the HSI measurements by taking the average of a 7 × 7 pixel region within the 100% dye spot locations for AR and NC.

As an example, if we were to look at the top right barchart, which corresponds to the 100% AR and 0% NC dye spot location, we see that the absolute error for LASSO and SPLASSO for AR the CAF is 0% (a result of normalizing against the abundance estimate at this location) and is approximately 10% for the SPLASSO and 40% for the LASSO for the NC CAF.

The (benz)indolium dye spotted glass slides were incubated with IQ-tag displaying phage and the binding was detected with anti-M13-HRP anti-M13-HRP anti-M13-HRP

The spectra obtained from the 100% dye spots were used to fit the remaining microarray spots (including the PEG spots), using a linear combination of the dye signals, and including a constraint of non-negativity.

To move from a qualitative to a quantitative characterization of ectopic projections would require significant effort and, ideally, the availability of raw image data would allow for various methods of determining the location of dye spots to be tested.

Fig. 3 shows a heatmap of the estimated abundances using the LASSO and SPLASSO for each of the two dyes (we have zoomed in on the dye spots and removed most of the background regions to ease visualization of the results) for the second of the three replicates (results are similar for the third).

The design and fabrication of custom-tailored microarrays [ 37] for use as phantoms in the characterization of HSI is described by Clarke et al. In this work, as the shape of the dye spots in the array results in significant scattering signals, which can be used to test image analysis algorithms, the separation of the scattering signals allows elucidation of individual spectra of different dyes.

The two totally discrepant cases were caused by partially folded TMA spot and falsely dyed spot.

While radioactive labels ensure high sensitivity and a linear range, they suffer from the problem of overspill, which is not experienced with conventional fluorescent dyes: spots with high intensity can outshine and erroneously contribute to the intensity of neighbouring spots.

Dye-swapping for each biological sample showed no dye-dependent spot changes on the gels (Additional file 4).