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Outstanding challenges for this type of display include generation of a photoactivatable red-blue-green (RGB) dye set for a true-colour 3D display, overcoming the challenge of 'ghost' voxels, optimization of optical slicing or photoswitch kinetics for versatile real-time 3D animations, and implementation into a solid-state device.
The samples were run on the ABI PRISM® 3130xl Genetic Analyzer (Applied Biosystems) using the Applied Biosystems Multi-Capillary DS-30 (Dye Set D) Matrix Std Kit as recommended by the manufacturer.
Additional file 14 lists information about the markers including the primers flanking the selected regions and the fluorescent dye set (Applied Biosystems, Foster City, CA) chosen.
The forward primer was 5′ end-labeled with one of four dyes in the ABI DS-33 dye set (Table 2).
PCR products were multiplexed (Table 1) and run on a 3130xl DNA Analyzer with GeneScan 500 LIZ Size Standard (G5 dye set) for fragment analysis (both by Life Technologies).
Photobleaching experiments were performed by using a Photonic instruments Micropoint laser system equipped with a 364 nm laser dye, set at 10 to 15 pulses with maximum laser intensity.
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The dyes are actually dye sets and are mixtures of various dyes with slight structural differences, each with a different glass transition temperature (Tg).
Although the top layer is essential for dye retention, it was demonstrated that the multilayering is essential not only for dye setting and dye capturing mechanism but can be utilized for vertically controlling the dye penetration and retention in the formed hydrophilic gel complex, which has a significant influence on optical effects and mechanical durability.
Four microsatellite loci— Ap-02, Ap-03, Ap-05 [ 71], and Aph10M [ 72] were PCR amplified with Dye Set-30 (DS-30) fluorescent primers using a touchdown reaction as follows: 94°C for 3 min; 45 cycles of 95°C for 30 s, 68 56°C touchdown for 13 cycles, then 55°C for 32 cycles, each cycle for 30 s, 72°C for 30 s; 72°C for final elongation, then held at 4°C.
The primer pairs were synthesised and fluorescently-labelled (Dye Sets DS-31 or DS-34) by Applied Biosystems, Australia.
The children's area is a tower of colorful crafts that range from baby-friendly crayons to a tie-dye set ($20).
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