Sentence examples for dye protocol from inspiring English sources

Sequencing was performed with ABI 3730 DNA analyzer, using a Big Dye protocol with Zymo column-purified products.

PCR products were prepared using a standard big dye protocol before sequencing using an ABI13730 capillary sequencer.

Differential protein expression was determined using 2D DIGE based QIP (Alban et al. 2003; Friedman et al. 2004) following a three-dye protocol that allows simultaneous labeling of an internal control and brain samples from exposed and unexposed rats.

20 µg of total RNA was labeled following an amino-allyl dye coupling protocol.

Mutation analysis was performed by direct sequencing using the Big Dye Terminator Protocol.

Under identical dye loading protocol the TMRM fluorescence in cells transfected with shRNA to Grx1 was significantly lower as compared to those transfected with empty vector and did not decrease further with time suggesting that the loss of MMP has already occurred (Fig. 3B,E).

All head kidney samples were analyses using the dye swap protocol.

We used KF, a 68-kDa fragment of E. coli DNA polymerase I, to develop our dye purification protocol.

Each template was sequenced using the Big Dye terminator protocol (Applied Biosystems) and analyzed on ABI 3700 and ABI 3730 sequencers.

Each of the 6 replicates was hybridized using a dye swap protocol resulting in 12 slides per group and 48 slides in total.

Top, diagram of dye loading protocol, showing the methods for assessing background dye loading under control conditions (no additional CO2), dye loading during a hypercapnic challenge (PCO2 55 mmHg) and the use of the hemichannel blockers to probe the identity of the hemichannel required for CO2 sensitive dye loading.