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These usually consist of several dye pellets, color coded cups, a special egg spoon, and, of course, directions to make the dye.
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One mL of a 0.5 M NaOH solution was added to the remaining collagen-bound dye pellet.
The tubes were allowed to gently mix for 30 min at room temperature before centrifugation at 15 000 g for 10 min The collagen-dye pellet was dissolved in alkali reagent supplied with the kit, and measured at 540 nm using Benchmark plus (Bio-Rad, Hercules, CA).
Effect of various physicochemical conditions on dye decolorization using pellets of potent fungal strain was assessed on flask level experiments.
The lab-scale reactor was developed and mineralization of Reactive Red 31 dye by fungal pellets was studied at 6, 12 and 24 h of HRT (hydraulic retention time).
Scale-up studies for RR31 dye degradation using fungal pellets was conducted in a designed and developed aerobic reactor.
The Egg Dyes: Skip the unholy mess of pellets and artificial food dyes.
After having removed the aliquots, the pellets were dyed with 200 μl (1.44 ml/100 ml Hank's buffer) acridine orange (Sigma, A6014-10G) foneone minute.
Labeling was performed by mixing 2 μL of DiOC6(3) (5 mg/mL) in the inactivated culture at 37 °C incubator for 30 min. Excess labeling dye was removed by pelleting and suspension in fresh PBS.
The increase in decolorization efficiency (94.51%) in the 2nd cycle may be attributed to acclimatization of fungal pellets towards RR31 dye.
The pellets containing the dye bound molecules were re-suspended in 1 mL of a solution of 40% dimethylsulfoxide (DMSO) and 0.07M MgCl2 then centrifuged at 12,000×g for 15 minutes and the supernatant was once again discarded.
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