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After this incubation, 10 µL of Redox Dye Mix MA (HepG2/C3A, HepG2, COLO 205, A549, PC-3) or MB (HL-60 and CCRF-CEM) was added, and cells were incubated to assess dye reduction for 5 hours (Redox Dye Mix MA) or 24 hours (Redox Dye Mix MB) and photographed.
Aliquots (100 µl) of cell suspension and 1× Biolog redox dye mix A were inoculated into each well of the plates respectively, followed by incubation at 28 °C.
Cell pellets were then re-suspended in 25-ml cold PBS, and 2 ml EB/AO (1 1, 4 mg/ml) dye mix was added.
The adherent cells were washed carefully with 1.0 mL of PBS followed by addition of 20 μL of the dye mix containing ethidium bromide (100 mg/mL) and acridine orange (100 mg/mL).
The overnight cultured bacterial colonies were inoculated into Biolog IF-0a GN/GP Base medium to reach 85%% turbidity followed by 1 200 dilution aliquoted into IF-10b medium supplemented with Dye Mix A as indicated by the manufacturer instructions.
The final concentration of dye mix was 4 µg/ml AO and 4 µg/ml EB in PBS.
Similar(22)
Red dye mixing experiments were carried out to visualize the development of mixing process and the performance in SOR-I and SOR-II.
The effect of a primer, as well as that of a black dye mixed with the rubber, was also investigated.
Further, the microfluidic paper-based analytical device (μPAD) of LCC on dye mixing gradient and pH gradient were developed with the characteristics, fast self-acting transportation and high-performance mixing of liquid flows.
In the dye mixing gradient the time cost was reduced from 2355 s in the solely-printed one to only 123 s in the five-stage of this LCC-μPAD.
Figure 8 UV Vis absorption spectral changes of Procion MX-5B dye mixed with WO3samples at different timings.a Rectangular slab WO3+ Procion dye,b nanowire clusters WO3 + Procion dye, andc rod-like WO3 + Procion dye.
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