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Localized absorption of a tightly focused beam propagating through an absorbing dye medium in PMMA produces different spatial distribution of temperature in the pristine and plasma-treated samples, and consequently, difference of spatial variation of refractive index acts as a thermal lens, resulting in severe phase distortion of the propagating beam.
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A box of hair dye, medium-brown.
The effect of various parameters such as catalyst dosage, concentration of dye, and medium on the photocatalytic degradation was examined.
Cells were harvested (trypsin 0.25%, 1 minute) every 15 minutes from 0 to 3 hours (time course experiments) or at specific time point following addition of the dye to medium.
After removal of the residual dye and medium, 100 ul of dimethylsulfoxide was added to each well, and the absorbance at 570 nm was measured using BMG microplate Reader (BMG Labtech, Inc., Durham, NC).
(go to a salon, bring in a photo of him from a magazine and just ask for that kind of style!) If you have blonde hair, just dye it medium brown.
After the plants were loaded with dyes, they were kept in dye-free medium for at least 30 min before starting the experiments.
The dyes showed medium to good substantivity which was detected by their exhaustion and fixation study.
When AG83 and GE1F8R parasites, preincubated with either free Rh 123 or PC-SA-Rh 123, were again incubated in dye-free medium, the intracellular Rh 123 concentration decreased with time regardless of the parasite strain (Figure 4C).
After staining, the samples were rinsed in dye-free medium for 20 min prior to testing.
In cell membranes exposed to dye-containing medium, bleached molecules are rapidly replaced by non-bleached molecules.
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