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Following treatment and once the fish had reached a mean weight of 28.4 ± 0.4 g, replicate treatment groups were identified by a coded subcutaneous dye mark and then reared communally.
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Success rates of fiducial marker placement, dye marking, and lymph node biopsies will be captured when applicable.
Virtual-assisted lung mapping is a novel bronchoscopic lung marking technique that uses virtual images to perform multiple concurrent dye marking of barely palpable pulmonary tumors.
Although each dye marking was located on the lung surface at the periphery of the branchial branch (i.e., inside of the segment), the appropriate intersegmental lines and the locations of blue sutures were determined based on the geometric information obtained from the "lung map".
More popular options include the Sea Dye Marker Packet and whistle.
Dye marks were located on 25 microns frozen sections stained with cresyl violet.
Squirrels were caught periodically to renew dye marks and assess reproductive condition.
Recording sites (blue dye marks) were localized by microscopic examination and then plotted on camera lucida drawings of serial sections for further analysis.
Histological sections stained with toluidine blue (dye marking cartilage proteoglycans) showed reduced staining of extracellular matrix and pronounced cartilage dysmorphology with accumulation of purple stained matrix within chondrocytes (Figure 1D).
However, proliferation per se is not the sole reason for the altered differentiation profile in the Id1 knockout cells since vital dye marking of the HSCs, which allows for marker analysis at each cell division, shows a clear change in differentiation status at each cell division.
The pH of the sample was adjusted to 8.0-9.0 8.0-9.0ther dye marking.
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