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Also, for all dye combinations combined (paired data), a confidence analysis was conducted.
* In bold the intersection of two gene lists indicating the modulated genes for a dye combination as percentage of all modulated genes (20 genes) found by analysis of all dye combinations combined.
* In bold the intersection of two gene lists indicating the modulated genes for a dye combination as percentage of all modulated genes (for 3, 10 and 30 μM: 121, 97 and 195 genes respectively) found by analysis of all dye combinations combined.
This combinational approach resulted in the identification of several dye combinations that recapitulate known invasion phenotypes, meaning that the assay can be adapted to multiple flow cytometers, depending on the laser lines available.
In italics the intersection of three gene lists indicating the modulated genes for two dye combinations as percentage of all modulated genes found for all dye combinations combined.
For all dye combinations, percentages of modulated genes relative to modulated genes for all dye combinations combined are generally equal (Tables 5 and 6).
Similar(37)
For confidence analyses, for each B[a]P concentration, data of the two replicate arrays with the same dye combination were combined.
A final dye combination with CY5 as reference dye and FAM/JOE/TXR as reporter dyes was selected.
The selection of the dye combination working on the ABI 7700 cycler for this multiplex PCR revealed crosstalk phenomena between several combinations of reference dyes and reporter dyes.
The availability of a variety of colored food and drugs, cloths, papers, and dye combination that are available in today's markets make the statement valid.
Therefore, the proposed dye combination and its corresponding protocol are deemed more suitable for use on complex environmental samples than the Live/Dead BacLight™ Bacterial Viability kit.
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