Acquisition of megapixel images with pixel dwell times down to the microsecond can be reached with negligible I/O transfer time.
An implementation of quick illumination sequences allowed us to realize up to 250-fold faster recordings as compared to previous reports, thus facilitating live-cell RESOLFT nanoscopy with pixel dwell times down to 70 µs.
Since the ER is a fast moving structure we increased the recording speed further by doubling the light intensities (on: 405 nm, 4 kW/cm², 20 µs; off: 491 nm, 20 kW/cm², 50 µs; read-out: 491 nm, 76 kW/cm², 5 µs) thus cutting the pixel dwell time down to 75 µs, which is ~250 times faster than reported previously using rsEGFP.
"The wolf also shall dwell with the lamb, and the leopard shall lie down with the kid, the calf, and the young lion, and the fatling together, and a little child shall lead them".
"I don't dwell on the negative," Down said.
You should find a way to slow that down and dwell in your apprenticeship and take pleasure in being playful and unfinished while you can.
" Well since my baby left me / I found a new place to dwell / It's down at the end of Lonely Street / At Heartbreak Hotel." Nigel turns to his band-mate.
At a time when Italy was experiencing its economic "miracle" and industrial cities like Milan were booming, arte povera slowed things down, to dwell on the stuff of life, the materials of the everyday.
For the dwell phase, the pump-down time can be significantly reduced, if all six cryopumps are utilized.
Average time in the yard, called dwell time, is down 7% to 23 hours.
