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As a result, during trains of action potentials calcium-induced fluorescence changes remain large in spines, whereas in dendrites overall fluorescence levels increase and changes become smaller rapidly.
Evidence supporting this conclusion has been obtained recently from electrophysiological recordings in rat somatosensory cortex during trains of whisker stimulation [28].
To examine differences in calcium dynamics in dendrites and spines during trains of APs, we applied short trains of five APs at 50 Hz during point imaging from spines and their parent dendrites (Fig. 1D).
We also measured synaptic depression during trains of HFS (20 Hz/2.5 s, Figure 3A).
With the muscle held at optimal length, the force developed during trains of stimulation pulses (i.e., tetanic contraction) was recorded.
In addition, activation of GC could also recruit feedback inhibition that could influence their activity, especially during trains of stimuli.
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