Exact(1)
To maintain the cloned HJO genome during this transposition reaction, the Red-mediated pBeloBAC11 insertion, the I-SceI-mediated double-strand break introduction, and the Red-mediated aphAI-mini-F fragment excision was induced in a novel synchronous reaction (for detailed protocol see Materials and Methods).
Similar(59)
Although errors during whole genome sequencing and assembly cannot be excluded [ 44], the cause of this transposition is difficult to infer.
You might say this transposition of imagination is the filmmaker's hallmark.
This transposition has not been adequately effected by individual governments.
This transposition did not occur in P1-rr1088 [ 17].
However, it is not clear if such a result is due to the tendency for a transposable element to insert itself into a similar class of repeat element during the transposition process (in this case, a DNA transposon inserted into another DNA transposon).
This would be analogous to another member of the RNase H/Integrase superfamily, Tn5 transposase, which interacts with the 5' phosphate exposed by hairpin cleavage during the transposition reaction [22].
A deletion is likely to occur between these two copies during the transposition process.
One potential source of error would be caused by mutations that occur during the transposition process itself.
In addition, the process of gene fragment capturing during the transposition of helitrons has not been characterized.
Mutations include deletions, insertions, and other type of structural variations that occur during the transposition cycle and after their integration into the genome.
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