Exact(7)
Viability of cells was maintained during this assay period.
It must be noted that none of the selection chemicals (i.e. E2, Tam or β-HCH) were added to the cell culture media used during this assay.
It was necessary to reduce the concentration of amino acids to one-sixth that normally present in α-MEM to enable detection of the flux of amino acids during this assay period.
With the use of a calibration curve, the amount of POPA generated during this assay could be read out from the intact membrane, an objective that has been otherwise difficult to achieve because of the lack of unique chromophores on PA lipids.
During this assay, all components being in a saturated condition, the only variation observed is exclusively linked to the concentration of visfatin/Nampt in the tissue extracts.
During this assay, we observed that ADCC was already presented using 1 μg ml−1 of antibody (comparison between the cellular death without or with 1 μg ml−1 of antibody of five tests was significant with P<0.05) and was still moderately increased with higher concentrations.
Similar(53)
It should be noted that during optimization of this assay, variations in results were obtained when different culture media were used.
The experimental artifacts that can complicate kinetic analysis using biosensor technology, such as heterogeneity of the ligand, mass transport, and nonspecific binding, were considered during the development of this assay.
With the addition of lower peptide concentrations during this titration assay, fewer pMHC complexes will be formed.
However, method validation predictions of the quantitative performances of the assay and estimations of measurement uncertainty may be far away from the real performances obtained during the routine application of this assay.
Since nonadherent dead cells are removed during the washing process of this assay, cells observed as deep blue, stained by crystal violet, account for viable cells only.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com