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This phosphorylation is essential for a variety of the connexin family members during their assembly into gap junctions [ 101].
However, the assembly of the complexes, especially of complex III, and the interactions between the complexes during their assembly is poorly understood.
In contrast, proteins with normal NC1 domains and with missense mutations in the triple-helix-forming domain are expected to be incorporated into heterotrimers during their assembly.
The methods we describe have complementary temporal and/or spatial resolution and thus provide information on different aspects of protein dynamics within and around adhesions during their assembly and disassembly.
Clusterin (CLU) is a chaperone protein of the uterine fluid, suspected to prevent the premature aggregation and precipitation of eggshell matrix components before and during their assembly into the rigid protein scaffold necessary for ordered mineralization [ 88].
We first measured paxillin dynamics in the cytoplasm, where it is present as single molecules or in very small aggregates, and then measured paxillin binding and unbinding to adhesions at various loci in the cell, including adhesions near the leading edge during their assembly and disassembly.
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"Essential decisions are made during their general assembly and are then carried out by the members invested with the responsibility to do so".
HLA class I antigens bind tumor-associated peptides during their intracellular assembly, which are then presented to CTL.
For the shorter rigid surfactants one observes gradual tilting during their self-assembly into bundles whereby the tilt angle increases substantially with increasing coverage.
The triplet 5'-TGT was identified at the termini of PaMx73 and H70 during their genomic assemblies (see Methods) and, except for the incomplete genomes mentioned above, the triplet was present in all the genome ends examined in this work.
The management of shapes during a design process and their assembly for a full aircraft are also tough tasks.
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