Sentence examples for during the transposition from inspiring English sources
This would be analogous to another member of the RNase H/Integrase superfamily, Tn5 transposase, which interacts with the 5' phosphate exposed by hairpin cleavage during the transposition reaction [22].
A deletion is likely to occur between these two copies during the transposition process.
One potential source of error would be caused by mutations that occur during the transposition process itself.
In addition, the process of gene fragment capturing during the transposition of helitrons has not been characterized.
Mutations include deletions, insertions, and other type of structural variations that occur during the transposition cycle and after their integration into the genome.
The results indicate that gene fragments captured during the transposition of many helitrons happen in a stepwise way, with multiple gene fragments within one helitron resulting from several sequential transpositions.
The problem is addressed by inducing the transposition during anther culture so that selected mutations can be transmitted and then regenerated to a homozygous state.
The most abundant IS element is ISSgl1, that belongs to IS5 family of IS sequences, with a functional transposase gene that encodes for a protein of 307 amino acids with a functional DDE domain common to most transposase genes responsible for co-ordinate divalent metal ions, mainly Mg+2, needed during the course of the transposition reaction [ 69].
To maintain the cloned HJO genome during this transposition reaction, the Red-mediated pBeloBAC11 insertion, the I-SceI-mediated double-strand break introduction, and the Red-mediated aphAI-mini-F fragment excision was induced in a novel synchronous reaction (for detailed protocol see Materials and Methods).
During the Mu phage infection step, various flanking sequences from the previous host are lost during the integrative transposition of the linear Mu DNA into a random site of the bacterial chromosome (Au et al. 2006; Bukhari and Zipser 1972).
There is no evidence for de novo formation of the transposition products during this period.
