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An efficient way of bulk loading is the incorporation of these molecules during the scaffold formation process.
Taking advantage of this impurity, 3D nanostructured scaffolds are built via layer by layer (LBL) deposition of HiPCO® SWCNTs and magnetic nanoparticles which are retained via magnetic interaction with the iron particle impurities during the scaffold formation.
Similarly, to achieve simultaneous addition of cells during the scaffold fabrication, novel robotic assembly and automated 3D cell encapsulation techniques are being developed.
The release studies showed a more controlled BSA delivery when the protein was incorporated during the scaffold preparation when compared to that where the protein has been loaded in a second step (ex situ process).
Therefore, future bone regeneration strategies utilizing scaffolds should consider scaffold architecture as an important factor during the scaffold optimization stages in order to move closer to a clinical application.
Since there is no overheating in the particles' internal region, the SSLS technology has the potential to maintain the nature of delicate biomolecules inside the polymer particles during the scaffold fabrication (Bartolo et al. 2008; Antonov et al. 2005; Kanczler et al. 2009).
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The Bovine Serum Albumin (BSA), employed as a model protein, has been included by using two drug-inclusion strategies: during the scaffolds preparation (in situ process) or by injection of an aqueous protein solution within (ex situ process).
With the ability of fully supporting its own weight of suspensions during assembly, the scaffold fabrication requires no sacrificial support material or mould (Smay et al. 2002).
The broad spectrum antibiotic ciprofloxacin (Cip) was impregnated to the scaffold during the fabrication in a concentration of 5, 10 and 20%.
In addition, it is important to determine the contribution of the forming tissue to the mechanical properties of the scaffold during culture to optimize the pore network architecture.
The conventional freeze-drying process for fabricating CG scaffolds creates variable cooling rates throughout the scaffold during freezing, producing a heterogeneous matrix pore structure with a large variation in average pore diameter at different locations throughout the scaffold.
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