Since mouse HSCs were maintained in suspension, no medium changes were performed between the two labelings, and a higher concentration of the second analogue was used in order to compete over the first one during incorporation into the replicating DNA.
A partition homogeneity test was done for 100 replicates, although the test was aborted during the 78th replicate due to time constraints (655:46 hr).
The profiles of the TS and VS in the replicate bioreactors during the successive cycles were identical (data not shown).
To ensure the fidelity of chromosome segregation of the replicated genome during cell division, the mitotic SAC delays the onset of anaphase until all chromosomes have made amphitelic tight bipolar attachments to the mitotic spindle.
Due to issues with a batch of samples during RNA preparation, one of the replicates from the following chemical stimulations had to be removed from analysis on all platforms: p-phenylendiamine, geraniol, eugenol and 1-butanol.
In addition to the samples removed due to issues during RNA preparation, one of the replicates from the following chemical stimulations had to be removed from analysis on BioMark HD™ and OpenArray® platforms due to insufficient amounts of cDNA: propylene glycol, methyl salicylate, geraniol and dimethyl sulfoxide.
PCNA has previously been shown to form distinct DNA replication foci in the parasites during replicating trophozoite stage.
Discrepancy between blood and nasal secretions is due to the relatively short period during which the virus replicates in the blood stream until immune response occurs.
Upon ORCA KD, there is a significant increase in the population of the locus replicating during early S (4-hr time point) and a concomitant reduction in BrdU ChIP signal at mid and late S (8 and 12 hr time points).
