Peptides were ranked based on the quantitation of tumour, kidney uptake and retention in the tumour during 4 h.
The quantitation was performed as described in the following.
The quantitation was performed using lisinopril as internal standard.
The quantitation wavelength was set at 245 nm.
The quantitation of total glucose in the culture medium allowed the estimation of cells' glucose consumption during the culture and was performed using a glucometer (YSI, 2700-D Biochem).
Bovine serum albumin was used as the quantitation standard.
Revealingly, the quantitation using MS2 mirrored more closely to literature values and the quantitation found in LC-DIA-MS, a methodology having widespread acceptance for reliable quantitation.
This signal saturation effect is overcome by LC-IM-DIA-MS2-High3 quantitation, demonstrated by the quantitation of abundant proteins in line with LC-DIA-MS quantitation.
Therefore, we showed only the quantitation for the untreated cells.
The quantitation was added to the stress granule data.
