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A stereomicroscope was fitted over the apparatus to view the fibre at 20 60× magnifications during the mounting procedure and during the experiment.
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The mounting procedure, rings vs. strips does not alter the behavior of the carotid media in tissue baths.
Problems with development may be aggravated by the dechorionation and mounting procedure.
List of basic materials and solutions that are needed for the clearing and mounting procedures.
Bright field imaging showed that a small portion of the cells detached from the slide surface during our manual washing and coverslip mounting procedures.
Collagen fibril arrangement and TEB structure is well preserved during the whole mount procedure and light scattering is reduced dramatically by extracting fat resulting in improved 3D structure, particularly for SHG signals originating from collagen.
A reactionless machine will not apply reaction forces and moments on the mounting base during motion.
No probes were damaged during insertion into, or removal from, the mounting device.
Assemble the mounting kit.
Prepare the mounting boards.
Fasten the mounting clips.
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