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Moulding technology was employed to achieve differences in heat transfer rates during the freezing processes.
The temperature distribution and temperature variations with time at different radial positions during the freezing processes were obtained.
The enhanced particle interlocking and ice strengthening during the freezing processes and the thawing-induced consolidation at the geometrical nonlinear regimes are both replicated in numerical examples.
Stem CO2 efflux during the freezing processes has not been reported before as far as we know, but it has been reported that stem CO2 concentration is affected by unknown drivers under freezing conditions (Etzold et al., 2013).
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The freezing process itself has no effect on nutrients, but during blanching (prior to freezing) water-soluble nutrients may be leached-out during the process (Bender, 1981).
This will speed up the freezing process.
Generating a temperature gradient during the freezing process resulted in unidirectional freezing, which aligned the formation of ice crystals during the freezing process and in turn produced aligned pores in CDM scaffolds.
The results show that controlled nucleation reduced the degradation of LDH during the freezing process, but this does not necessarily translate to vastly superior stability during the entire freeze-drying process.
To protect the proteins during the freeze-drying process, the purified malleins, retentate of the TFF process, were mixed with equal volumes of a buffered 14% glucose solution [ 26].
As such, cryopreservatives have been employed to retain viability during the freezing and thawing processes.
However, since TMR was not reported for fresh semen, it is not possible to know how well the test would reflect changes which have taken place during the freezing and thawing processes.
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