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Nonadherent, haematopoietic cells were removed during the first medium replacement after 3 days in culture.
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and AR is the first medium that is completely unbound.
In contrast, the cells from the SNCA_Tri line showed a tendency to migrate and form local clusters during the first days of DA2 medium cultivation (see day 4 in Supplementary Movie 1), after which some cells continued to proliferate while those with an incipient neuronal appearance failed to develop a complex network.
(1) At the time of Sidon's foundation, during the third millennium BC, medium sand facies show the city's northern and southern pocket beaches to have served as proto-harbours for Middle to Late Bronze Age societies.
The decrease in PLGA molecular weight during the first days in the release medium, despite of being minor, significantly conditioned hGH release rate.
A total of 1189 yeast strains were tested in test tubes containing 3 mL of mixed-sugar medium during the first screening step.
To better appreciate the role of ERK activation in the regulation of the myogenic program, C2C12 myocytes were exposed to TNFα and PD during the first 48 h in differentiation medium.
Both proliferation potential and viability were also investigated in those BMMCs which were suspended in the cell medium during the first 3 days of culture.
Our preliminary experiments showed that autophagy of AF cells could hardly be detected during the first 12 hours culture in medium with 10% FBS.
The reactors working volume was 1 litre and the total SBR cycle duration was 12 h, consisting of 10.5 h of aerobiosis, 1 h of settling (agitation and air sparging switched off) and 0.5 h to withdraw half of the volume, which was replaced by the same volume of fresh medium during the first 15 min at the beginning of the next cycle.
The unattached BMMCs which remained suspended in the medium during the first 3 days of culture were also able to subsequently adhere to the coated dishes and expand; thus, we suggest that the amount of BMMC-derived EPCs can be further increased by simultaneously growing both early-suspended and adherent cells under the same culture conditions.
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