Also during the experiment, EMG activity of the resting limbs was closely monitored by the subjects and the experimenters and trials were repeated when overt EMG activity was observed.
General status of rats was observed during the experiment, including activity, breath, fur, especially mouth and nasal secretion, breath conditions and food and water intake.
We evaluated several other variables as potential confounders, including water consumption, source of water, antioxidant intake from diet, number of laps swum during the experiment (an indication of physical activity), and leisure-time physical activity.
Eighteen were excluded before analysis as they reported engaging in a distracting activity during the experiment, leaving a final sample of 160 (80 women, mean age = 36.4 years; SD = 12.2 years).
recorded the animal's activity during the experiment using near-infrared illumination.
Importantly, oral treatment with GGA did not cause any adverse effects and the mice exhibited normal BW gain and activity during the experiment.
Duplicate sterile controls were included, which were autoclaved for 1 h at 121°C, followed by addition of 50 mg/l HgCl2 to prevent biological activity during the experiment.
The PicoGreen assay did not reveal any proliferative activity during the experiment, which rules out the possibility that adhesion differences between drug resistant, VPA-treated and control NB cells may be caused by different cell growth capacity.
These results may indicate the slow copper precipitation process under neutral conditions (pH fluctuated around 7.5 ± 0.4 during the experiment) and absence of microbial activity connected with the leaching of copper.
CO2 production, redox potential and microbial number were monitored simultaneously in the aquifer during the experiment to assess the EMs' activities and the effect of the bio-barrier.
