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The formation of these structures may be of relevance during the entry process into these vessels.
Protein H is a multifunctional structural protein (12 copies per virion) required for piloting the viral DNA into the host cell interior during the entry process [10].
In addition to the receptor-binding mediated conformational change of viral glycoprotein, sequential conformational changes induced by proteolysis are required during the entry process of several viruses.
Some recent studies suggest that cellular MT motors catalyze the intracellular transport of viral core structures during the entry process of both DNA and RNA viruses; examples are herpes simplex virus [5], human cytomegalovirus [6], parvoviruses [7] [8], human immunodeficiency virus [9], influenza A virus [10], and vesicular stomatitis virus (VSV) [11].
For productive infection, both EV membranes must be removed during the entry process.
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Having established the fractions that contain early and late endosomes, we purified endosomes via a sucrose flotation gradient to quantitatively track KSHV in infected HFF cells post-internalization and to characterize possible role(s) for DLD-binding integrins during this stage of the entry process via qPCR (Figs. 1, 2C).
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This mAb recognizes a highly conserved epitope in the HR-1 trimer and it is more effective as an Fab fragment as compared to intact IgG, a fact that is may be explained by the limited accessibility of the epitope, which is likely to be exposed only transiently on the cell surface during the viral entry process [70].
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