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Trace amount of p-coumaric acid was also detected during the cultivation process (at 24 h) of the strain ST4982, but not in the other strains.
Rhamnolipids were highly concentrated in the foam during the cultivation process with enrichment factors up to 200.
To determine the fungal biomass, 25%% of the solid substrates (SS) were labeled using small holes in different distributions for possible monitoring during the cultivation process.
Foam formed during the cultivation process was collected via the exhaust cooler in single use bags that were cooled on ice to prevent further growth of foamed microorganisms.
In addition, it was observed that peptone induced filamentous forms of mycelia during the cultivation process, whereas the other three nitrogen sources induced the formation of fluffy pellets.
Foam-fractionation, the separation of foam via an outlet of the reactor during the cultivation process has been successfully applied within the production of biosurfactants (Chen et al. 2006; Davis et al. 2001; Willenbacher et al. 2014) and not only hinders the increase of pressure in the reactor but also displays a first step of product removal.
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As a result, no phosphorus source was added during the whole of the cultivation process in this study.
The cultivation process, however, needs to be repeated until the seed bank is depleted.
Mold, pesticides and other chemicals used in the cultivation process can also bring as yet undetermined negative effects.
The cultivation process in FAP-free medium was referred as "G0".
The cultivation of aloe vera should be organic, so during the entire cultivation process you should not use any fertilizer, pesticide or herbicide chemical.
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