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The compositional variability is interfered from uncertainties introduced during the analysis subject to the analytical method chosen.
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The strength of this procedure is that during the analysis subjects are not clustered a priori, e.g., in categories as patients and controls.
Further smearing is a result of group-level averaging of activity across all the subjects during the analysis.
The coding framework was subject to continuing iterative revision during the analysis.
To solve confidentiality issues, all subjects were assured that personal identification, including names, would not be disclosed during the analysis, interpretation, and publication of data.
Importantly, and in order to add confidence to the RT-PCR results, these animals were different to those used during the microarray analysis but were subjected to the same wetting regime.
Additional subjects were removed from the analysis during data checking and are described in Figure 1.
None of the subjects included in the analysis changed their medication regimen during the entire study period.
All 18 subjects were included for the analysis performed during wakefulness.
Data obtained during the experiment were subjected to analysis of variance.
To examine the effect of the experimental manipulations on the pupillometric data, pupil activity was averaged within specific time windows during the trial and subjected to analysis.
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