Sentence examples for during temperature shift from inspiring English sources

Interestingly, contrary to expectations, the transient overexpression of miR-7 led to growth arrest, resulting in increased recombinant protein production, generally observed during temperature shift culture conditions.

Consequently the yields of Y3 and Y4 were higher than that of the constant temperature culture (at 30 and 35 °C) during the temperature shift from 30 to 35 °C.

This characterizes cells in late mitosis or G1 phase and this analysis identified cells that had not gone through cytokinesis during the temperature shift.

To ensure that our results in the different cell types were not influenced by reduced cell survival during the temperature shift or transfection procedures we used trypan blue stainings on all cell types to proof cell viability.

In a similar approach, Barron et al. (2011a) identified miR-7 as significantly down-regulated during a temperature shift from 37 °C to 31 °C.

Recently, this protein has been shown to be upregulated during a temperature shift similar to that experienced by the parasite upon transmission from the insect to the vertebrate host (see Table  3 in [ 67]).

To elucidate the mechanisms of heat resistance, we analyzed the change of the A. fumigatus proteome during a temperature shift from 30°C to 48°C by 2D-fluorescence difference gel electrophoresis (DIGE).

During this analysis, two known growth inhibitory miRNAs, miR-21 and miR-24 were identified as being upregulated during stationary phase growth induced either by temperature shift or during normal batch culture by both bioarray and qRT-PCR.

Because each HPV-specific pitcher will hybridize with a catcher of a distinctive length causing a temperature shift during the melting out, different HPV types will be detected in one single channel using one fluorophore.

Conversely, simulations of glucose-limited conditions revealed strong changes of intracellular metabolite concentrations during the temperature shifts.

While the Hsp genes are commonly identified as overexpressed in short-term temperature manipulation experiments [ 24, 37], they are less likely to be targets for selection during gradual temperature shifts associated with climate change [ 22, 53].