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For the multilayer samples, 0.8 nm Ge was deposited at 530°C in each layer, which was separated by ~10 nm Si spacer with ramping substrate temperature from 500 to 530°C to suppress Ge-Si intermixing during spacer growth.
Direct evidence was found for transpositional activity occurring during spacer acquisition.
PCR analyses of the OrfB location before and during spacer acquisition indicated that this OrfB element was gradually lost over a 12 day period, suggesting a link between spacer acquisition and transposition.
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Gap tensioning was done during the spacer surgery, so there was no need for extensive scar release during the re-implantation procedure, which resulted in shorter operation time.
The G might be added during the spacer acquisition process, rather than by replacement mutation after the spacers' insertion into the CRISPR locus, in which case we would expect to observe the two variant spacers in the population, as seen in sets 11, 12, and 13 (Table 1).
We have developed an Altef tapered aluminium mold for production of a custom-made PMMA spacer during intervention.
Most of the two-stage exchange algorithms recommend the implantation of an antibiotic-impregnated spacer during the first stage for a period of 2 24 weeks before reimplantation of the new prosthesis [ 3- 5].
The sonication culture is a complementary method that can help to discover the possible persistence of microorganisms on an antibiotic-loaded cement spacer during a two-stage exchange protocol.
Most of the two-stage exchange algorithms recommend the implantation of an antibiotic-impregnated spacer during the first stage for a period of 2 24 weeks before reimplantation of the new prosthesis.
Interventions 200 μg extra fine hydrofluoroalkane (HFA) beclometasone dipropionate twice daily or matched placebo administered by a pressurised metered dose inhaler and a spacer during the first three months after hospital admission.
DOI: http://dx.doi.org/10.7554/eLife.08716.013 We next checked for specificity at the 3′ end of the 5′ flap in the disintegration product, which corresponds to the 3′ end of the incoming spacer during integration.
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