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In short, differential gene expression was calculated using FPKM values for every gene while incorporating expression level variances during significance testing.
In order to expand differential expression lists to facilitate functional analysis, the stringent multiple test correction was removed during significance testing, and transcripts that showed any expression difference were included in this analysis (P ≤ 0.05).
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If under-represented in the processes examined, these genes would introduce negative bias into the null distribution (and therefore increase the number of statistically significant outliers) when randomly sampled during bootstrapped significance testing.
Bias minimisation methods used during clinical significance assessments generally included a review by independent clinical pharmacists or the achievement of consensus among the ratings of clinicians, nephrologists and pharmacists.
Leaf initiation is not significant to rainfall during current months and had a strong negative significance during a 3-month lag period (rs = −0.70, P < 0.0003) (Figure 2).
Irvine detectives had missed their significance during their investigation, as had Duff during the first trial.
For example, SlGRAS38, SlGRAS35, and SlGRAS47 display relatively strong and specific expression during fruit ripening, indicating that they might have functional significance during the onset of ripening.
After 24 weeks of treatment the increase in RBF and the decrease in the WLR reached statistical significance during treatment with vildagliptin, but not during treatment with glimepiride.
Although increased activity can be observed in the light phase during all L : D conditions, this increase reached statistical significance during the longer photoperiod cycles (Zadjusted = −2.24, P = 0.02, Figure 4).
Then adjusted analysis was performed by entering variables that have shown significance during univariate analysis or have been previously reported to be significant in other literatures.
E-selectin was lowered with borderline significance during atorvastatin treatment (-7%, p = 0.11), E-selectin reduction during fenofibrate therapy was statistically significant (-10%, p < 0.05).
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