Moreover, we performed these experiments, as well as serum cytokine measurements, leukocyte subset analyses and serum free light-chain analyses, on blood samples collected during a symptomatic episode, anakinra treatment, and at several time points during a trial with canakinumab [ 13] in order to investigate disease-specific characteristics and the effect of IL-1 on these markers.
To estimate the "initial rate" (v 0 ) of the GSs reaction, the production of hydroxamate was measured at several time points during 15 minutes and exhibited a linear increase during the first 3 minutes for all four isoenzymes (data not shown).
Control organisms were sampled at several time points during embryonic and larval development until 33 days post-fertilization.
Membrane samples were imaged by scanning electron microscopy at several time points during in vitro degradation to assess any changes in pore structure.
Levels of BOEs for ten selected cigarette smoke constituents (nicotine, carbon monoxide (CO), benzene, 1,3-butadiene, acrolein, hydrogen cyanide, crotonaldehyde, 4- methylnitrosamino -1- 3-pyridyl -1-butanone [NNK], pyrene, 4- methylnitrosamino -1- 3-pyridyl -1-butanonemeasured at several time points during the study period.
We experimentally ablated the precursor cells that normally give rise to male genitalia at several time points during late larval development and examined the degree of horn development in these males compared to that of untreated and sham-operated control males.
To determine the optimal cycle length, cell numbers were counted at several time points during primary and secondary expansion cycles.
By drawing composite immunological profiles of the different infection groups, our analyses demonstrate that these profiles do indeed distinguish groups at several time points during the first weeks of infection with virulent FIVC.
Ten µl aliquots were transferred into an anaerobic chamber at several time points during a 24 hour period and inoculated into 90 µl of absolute ethanol or sterile deionized water.
Aliquots of the spores in either sterile deionized water or germination solution were also transferred into an anaerobic chamber at several time points during a 24 hour period and quantitative cultures were performed.
However, the increase of explorative activities shortly before the light-dark transition was much more pronounced and advanced by some hours in HR mice compared to the other two lines, resulting in significant differences at several time points during the light phase (KWH-tests: N = 12 for each line, experimental time points: 6, 7, 8, 9, 10, H = 6.1 12.6, df = 2, all p<0.05; see Fig. 3).
