Exact(4)
Therefore, the inconsistencies between dye-labeled assays are likely due to labeling efficiency or errors during quantitation of the dye signal.
β-actin was amplified in each set of PCR reactions and this gene served as internal references during quantitation to correct for operator and/or experimental variations.
Group A samples were classified as "below 48 copies/ml" for those with an observable amplification of virus during quantitation and Group B were classified as "nondetected" for those for whom no amplification was detected.
Therefore, we applied the following rules during quantitation: (i) voids were sharply bounded hypointense objects of round shape; (ii) voids needed to feature an intra-parenchymal localization; and (iii) to avoid partial volume effects, voids needed to exhibit at least a distance of one diameter of their own size from specimen surfaces or ventricles.
Similar(56)
The data were analyzed before and after manually reconfirming any peak with a coefficient of variation (CV) of >40% (48 pairs, 96 total metabolites); this procedure is referred to as "polishing" in Table 1, and was done specifically to determine if the analytical error originated before or during the HPLC analysis or during peak matching and quantitation.
Despite the limitations imposed by the matrix in question, the accuracy results and total error calculations generated during method validation revealed the consistency of quantitation across the entire quantitative range and compare well to the biomarker field (26).
Immunoblotting with the general TFIIB antibody demonstrated equivalent immunoprecipitation of TFIIB regardless of the presence or absence of phosphatase inhibitors during the lysate preparation (quantitation is shown below the autoradiogram).
Among 31 women treated for trichomoniasis, the concentration of vaginal HIV-1 RNA was above the limit for quantitation before, during, and after T. vaginalis infection in 4 (13% [95% CI 4% - 30%]), 4 (13% [95% CI 4% - 30%]), and 5 (16% [95% confidence interval {CI} 5% - 34%]) women respectively.
Other possible reasons for read variation include different percentages of poly A+ in different samples, differential efficiency of conversion of RNA to cDNA, size or GC composition variation among libraries (smaller and/or lower GC content amplicons amplify more efficiently during PCR for library quantitation).
Quantitation showed that during cooperative invasion, cells were found predominantly on these collagen and fibronectin tracks.
VRI allows a non-invasive quantitation of airflow distribution during different modes of mechanical ventilation.
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