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The catenated activity is resolved into two protein components during purification.
The latter is known to be only loosely bound to SecYE28 and was apparently (at least partially) lost during purification.
DNA remained in total RNA was depleted by On-Column DNase I Digestion Set (Sigma) during purification.
Shale brine is also associated with high levels of bromide, which is not toxic by itself but turns into carcinogenic trihalomethanes during purification treatment.
The kinetics of PvDHFR carried a larger error than HsDHFR because PvDHFR was prone to loss of activity during purification and storage.
Increased product concentration in the final solution resulted from the optimized process would reduce the downstream steps during purification.
The protein's extreme stickiness and susceptibility to proteolysis require careful attention during purification and in tropoelastin-based assays.
The New Light of Myanmar, a government-run daily newspaper, reported that during "purification" searches at 18 monasteries, the authorities had found, among other things, pornographic videos, "one Nazi headband and two American headbands".
Furthermore, unexpected defects or contaminants could be introduced into the CNM during purification procedures.
In both cases, no monomeric diabody was detected during purification or subsequent size exclusion HPLC analyses.
The concentration of protein during purification studies was determined by Bradford method [14].
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CEO of Professional Science Editing for Scientists @ prosciediting.com