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The effects of cool-down temperature during patch bonding, disbond and bending moment were also investigated.
To remove artifacts on the patch edges we set overlap of one pixel during patch extraction from the image.
Residual thermal stresses, developed during patch bonding, requires the knowledge of temperature at which adhesive becomes effective in creating a bond between the specimen and patch.
Further, the bending moment and temperature change during patch curing have a relatively more effect on the out-of-plane deformation than on the in-plane strain of the repaired panel.
LMNs were identified during patch clamp recordings using an inverted stage microscope equipped with epifluorescent optics and rhodamine filters.
We also employed electrophysiological assays to explore the potential for acute post-translational modification of ion channels during patch clamp experiments.
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Biocytin infused into cells during patch-clamp recordings in acute slices revealed that processes of these cells were largely confined to the injection tract (Fig. S3B).
(C ) Summary data of dendritic territories covered by W3-RGCs labeled biolistically or filled during patch-clamp recordings in WT (black ) or VGluT3 −/− (blue ) retinas.
Selective photostimulation of interneurons during patch-clamp recordings of either NG2 cells or pyramidal neurons was obtained with 5-µm illumination spots during 3 8 ms and a laser power of ∼12 mW under the objective.
During patch-clamp experiments, valleys and voids are filled with conductive media facilitating ion escape, increasing the leakage current and compromising the seal.
The resulting shuddering, lurching and ever-changing perception of gravity is something similar to the feeling you get sitting in an airplane during a patch of heavy air turbulence.
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