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Control cells were subjected similarly without the addition of E-cadherin antibody during labeling.
By using a metabolic labeling approach that can easily be adapted to other viruses, we have bypassed the requirement for high concentrations of reducing agents during labeling.
AMCA-HPDP was used rather than biotin-HPDP during labeling to allow S-nitrosylated proteins to be imaged in-gel prior to staining total protein; this aids in normalization of fluorescence against protein mass in each band.
Thus, the decreased expression of mTNF reported in Figure 8 cannot be explained by competition during labeling.
Because of the lysine content of CPPs, this lysine residue was replaced with a cysteine (Cys-ELP) in these constructs to provide selectivity during labeling reactions.
We anticipate having depolarized mitochondria in the preparation used here because we used mechanical homogenization, which is a harsh cell disruption procedure, and vortexing at 300 rpm to mix isolated mitochondria with JC-1 during labeling.
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Results from this assessment indicate that with appropriate measures to mitigate spray drift to shallow water bodies, applications of chlorfenapyr do not present a hazard to aquatic organisms during labeled uses.
To prevent this dye bias, we routinely swap the dyes during labelling for repeated hybridisations.
The addition of 0.05 2 mM ATP did not protect MsbA from this small activity loss during labelling.
Of those tested, fluorescein diacetate 5-maleimide (Fig. 1B) gave significantly less protein loss by precipitation during labelling.
We used three BFA protocols: we added BFA (i) before the start of [C]proline labelling in a pulse chase experiment, (ii) at the start of labelling, or (iii) during labelling.
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