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With just a minimum of prior information on expected homologous compounds to confine this detection, the presented algorithm will reveal series regardless of their specific ionized species, certain modifications during ionization or nonlinear RT properties.
However, such a less specific MS method can work only if no [M − 1]+ ion is generated during ionization.
Some possible reasons included the higher injection volume used in LC-ESI-MS/MS (5 vs. 1 μL), the lower amount of fragmentation during ionization (ESI vs. EI) [14], etc.
The examples discussed thus far are from data generated from electron ionization (EI) GC/MS analyses, where all of the ions detected are a result of fragmentation of the intact molecule during ionization.
Although the full band Monte Carlo simulation shows the effects of transient velocities and dead zones required by energy conservation during ionization, there is still a reasonable agreement between the two simulations.
This novel method uses MS data of characterized glycopeptides to analyze glycosylation profiles, and several quality control tests were done to demonstrate that the method is reproducible, robust, applicable to different types of glycoproteins, and tolerant of instrumental variability during ionization of the analytes.
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The most abundant ions will attract the most charges during electrospray ionization, making it less likely for low-level peptides to get ionized.
Previous study has verified that the binding of Cyt c with Mal II did not arise from the nonspecific interaction during the ionization in the ESI MS [10].
In GC/MS or LC/MS, many ions may be produced from the same metabolite during the ionization and fragmentation processes.
Signal intensities from S are due to the SO4 2− anions present during the anodic process, and Ar traces are provided for the trapped gas during plasma ionization, which are also clearly revealed.
This work serves as a follow-up to Part I of experiments designed to determine the underlying principles in the formation of pseudomolecular, or adduct, ions during electrospray ionization.
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