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Isogenic transplantation assays done some years before freezing and immediately after thawing indicated no measurable loss of clonogenic cells during freezing, storage or thawing; the number of cells required for 50% successful transplantation remained close to unity in both cases.
One possible explanation might be linked to platelet storage-derived lesions: it has been reported [ 28] that during freezing storage platelets decrease in their responsiveness to aggregating agents and thus appear to reflect a general loss in the ability to become activated by various agonists.
Maternal thyroid function during pregnancy was assessed using FT4, TSH, and TPOAb, all of which have been shown to resist deterioration during freezing, storage, and thawing (Mannisto et al. 2007).
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As sub-lethal injury of bacterial cells may occur during frozen storage, the samples were initially thawed over a 24 h period in a refrigerator at 4.0 ± 0.7 °C in order to enhance the recovery of the contaminating bacteria before the analyses were performed.
In practice the more severe cases of drying occur during frozen storage rather than during freezing.
This adds to the changes in texture and flavour occurring during frozen storage.
For freezing, arrange in a single layer, then stack frozen layers into plastic bags for longer freezing storage.
AFLP genotyping was used to differentiate 60 C. jejuni isolates, comprising 29 of 32 C. jejuni isolates from herd HH (three were lost during freeze storage) and 31 clinical isolates obtained from human patients in the study area during 2000.
Some transfer of moisture from the product is unavoidable during freezing and frozen storage, which leads to dehydration of the fish.
The position of the extreme points on the temperature axis and the absolute magnitude of the reaction rate were controlled, along with other factors, by the freezing mode used, i.e. by the thermal history of the system during its transition to the frozen storage conditions.
S. thermophilus was more resistant than L. bulgaricus and the cryoprotective effect of glycerol during freezing and storage was confirmed.
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