Exact(3)
It is generally accepted that, during first passage, LPs from RIII mice can be used to discriminate BSE from scrapie (5 ), though this principle has been challenged (22 ).
For secondary passages, brain from a mouse that succumbed to TSE during first passage was used to prepare a 1% (w/v) homogenate in sterile saline.
From a method perspective, the data suggest that AR, IP, and LP are not optimal bioassay parameters for differentiating TSE sources during first passage because they represent mean values derived from a group of animals that have been inoculated with a specific source.
Similar(57)
The primary requirement of radionuclide imaging is that the bolus of radionuclide should remain within the blood vessels during its first passage through the right and left sides of the heart.
This double-digestion process enables rabbits to utilize nutrients that they may have missed during the first passage through the gut and thus ensures that maximum nutrition is derived from the food they eat.
Most of the drug is metabolized by the liver during its first passage through the portal circulation resulting in 25% oral bioavailability [39].
Maryland law permits falconers to capture immature hawks during their first passage south for the winter, which is when many of them die because food is scarce.
"Thus it offers us a novel opportunity to see how the dust and gas frozen in this comet since the dawn of our Solar System will change and evolve as it is strongly heated during its first passage close to the Sun".
Normal human melanocytes were isolated from newborn foreskins and grown in OptiMEM (Invitrogen, Carlsbad, CA) with antibiotics, 5% fetal calf serum (regular medium) and growth supplements [53] and used during their first passage.
One additional blind passage was performed if CPE was not observed during the first passage.
Virus was isolated from tissue samples in a Vero cell line during the first passage.
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