Exact(4)
Oxygen consumption (VO2) and energy expenditure were significantly increased during fed and fasted states in mice treated with PF-879 and PF-879 plus GW501516 compared to vehicle.
More recently, P. pastoris strains producing an insulin precursor were analyzed for changes in the cellular proteome as adaptation response to methanol induction during fed batch cultivation using 2D-DIGE and subsequent mass spectrometry identification of differentially abundant proteins.
Since there was strong evidence that (partially) unfolded product was retained inside the cells during fed batch cultivations [ 4, 7], thus leading to an upregulation of the UPR marker protein BiP, we investigated the behaviour of these proteins also in the chemostat.
Although driving not only glucose and amino acid catabolism, but also fatty acid oxidation in times of energy deprivation [ 14], similar respiratory exchange ratios both during fed ad libitum and fasting periods rule out an impaired fatty acid utilization in absence of Sirt3.
Similar(56)
A pH-stat control strategy was utilized to investigate the optimum carbon source amount fed during fed-batch cultures for higher cell density.
Whereas the highest biomass concentration was observed during fed-batch fermentation, the maximum resveratrol production was achieved in batch mode.
A linearized stability analysis, supported by numerical illustrations, reveals that some or all of the limit points may be accessible during fed-batch operation.
In addition, compared to the batch culture, a much higher maximum hydrogen production rate (102.33 ml H2/l h) was achieved during fed-batch culture.
Peak power values of Gel-Lay (133.8 184.6 μW) during fed-batch cycles were also higher than the control (133.4 160.5 μW).
The constructed strain accumulated up to 16.4 g/L of riboflavin in optimized medium in a 7 L laboratory bioreactor during fed-batch fermentation.
In conclusion, Mrl2, a new laccase from M. roreri was identified and showed exceptionally high expression levels during fed-batch fermentation of recombinant P. pastoris (280,000 U l−1; 1.05 mg l−1) matching those of filamentous fungi.
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