Sentence examples for during different passages from inspiring English sources

Thus in using these cells, we can be assured that the cells are myofibroblasts, and we have previously published the characteristics of these cells during different passages.

Immunohistochemical expression analysis of the different key metabolic proteins was also performed using epithelial organotypic rafts cultures obtained from low passage keratinocytes expressing HPV16 E6 and/or E7 expression, as well as from PHK transfected with HPV16 or 18 full-length genomes and grown during different passages before raft culture seeding.

This could be due to recombination events occurring during different passaging in cell culture or to a decrease in sensitivity of the used assay with increasing copy number of target sequences.

Some other markers are present at the beginning but do disappear or are lost during the different passaging while other will increase, as passaging will select more homogenous population with more homogenous cell surface markers [ 153– 153].

The OSA-05 cell line was repeatedly demonstrated as nestin-negative during the long-term cultivation at different passages, and this cell line was derived from the only telangiectatic osteosarcoma, in which the only rare occurrence of Nes+ cells was demonstrated.

We also note that despite the known genomic instability of TNBCs [ 68], we observed that all PIK3CA sequence variations persisted between patient primary tumors and xenograft models, and between xenograft models assayed during different sequential passages.

Immunophenotyping on different passages were carried out as described above.

Experiments were repeated in 3 different passages of cell line.

Genetic profiles were very similar not only between the patient tumour and the xenografts, but also when comparing xenografts at different passages, suggesting that although extremely altered, the HBCx-17 xenograft genetic profile was stable during subsequent passages.

Both cell lines had subpopulations in different passages.

The relative state of differentiation is also retained on passaging (two different passages between P6 and P12).