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Cells were voltage clamped during continuous perfusion at room temperature.
Tubules were incubated with 5 μ m basolateral BCECF AM in control solution for 20 min at RT during continuous perfusion with control solution, followed by a 5-min washout period.
For comparison cilia beat frequency during continuous perfusion of standard external solution (aCSF 3 K+) was 9.8 ± 1 Hz (0 1 s), 10 ± 1 Hz (15 16 s), and 10 ± 1 Hz (29 30 s).
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Her haemodynamic status remained stable under continuous perfusion of norepinephrine during and after SAE.
Finally the cells were stimulated again with a continuous flow of epinephrine solution for 30 min. The three independently operated inlets allowed us to perform continuous perfusion of cells during the assay without any abrupt changes of shear stress or laminar flow perturbation inside the microfluidic chamber.
During the thoracic wound closure, a continuous perfusion of 0.2% ropivacain (6 to 8 ml/h) will be started.
In addition to the continuous perfusion, boluses were allowed every hour, particularly during the nursing care.
To describe sedation with continuous perfusion of propofol in critically ill children.
Cell seeding in continuous perfusion bioreactors resulted in more uniform MSC distribution than static seeding.
Pre-formed spherical microtissues are loaded into the microchambers and cultured under continuous perfusion.
BL concentrations were measured at peak and trough for intermittent and extended perfusion, and at plateau for continuous perfusion, by high performance chromatography coupled with mass spectrometry.
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