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Results indicate that glycerol as a dry product can replace dietary starch as corn starch at a level of up to 8% of DM in the diet without negatively affecting ruminal fermentation and digestibility during continuous culture.
Replacing corn starch with dry glycerol also linearly increased the digestibility of dietary neutral detergent fiber without a change on the flow or efficiency of bacterial protein synthesis during continuous culture.
Samples were removed from the chemostat once every generation during continuous culture.
Culture samples were harvested from the chemostat in the batch phase and during continuous culture and used to isolate genomic DNA.
The stability of the sorted cell populations was investigated during continuous culture for a period of three months.
It has long been known that during continuous culture of Tetrahymena thermophila, macronuclear telomeric sequences in vegetatively dividing cells are added and removed, a process thought to be regulated by cellular signals (Larson et al, 1987).
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A medium for continuous operation was also designed and, using that medium, demonstrated that cell concentration and interferon-α production increased proportionally according to glucose concentrations in the reservoir during the continuous culture (dilution rate, D = 0.2 1/h).
Thus, aberrant chromosomal segregation induced by DNA lesions might generally cause chromosomal alteration with the resulting loss of genomic homeostasis, which is also consistent with the observation of chromosomal loss in association with M-phase DNA lesions during the continuous culture of the immortalized MEFs (Fig. 7B).
Despite the lack of significance in the trend of changing productivity during the continuous culture, there was a statistical decrease in the flux from amino acids towards the synthesis of HCG.
Further characterization of the strain indicated that the selection pressure during prolonged continuous culture in xylose and arabinose medium resulted in the improved transport of xylose and arabinose as well as increased levels of the enzymes from the introduced fungal xylose pathway.
These genes were not induced in continuous culture during steady-state growth under low oxygen conditions (0.2% DOT) in our previous study [ 1].
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