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The chondroitin 6- to 4- sulfate ratio decreased steadily during construct maturation.
Future studies should determine the effects of temporal HP application during construct development, as well as examine the immediate and long-term effects of HP application on construct properties.
Fluorophore-assisted carbohydrate electrophoresis (FACE) analysis was additionally performed on Phase II samples (n = 6) to quantitatively determine changes in CS-4, CS-6, glucose, dermatan sulfate, and hyaluronan during construct development, as previously described [13], [23], [24].
Using a scaffoldless cartilage tissue engineering model [19], [20], this study sought to test the hypotheses that 1) a short-term application of static HP during construct development will have the greatest enhancement of construct biochemical and biomechanical properties, and that 2) there will be additive or synergistic effects when combining growth factors and HP stimulation.
Last but not least, defining a minimal, clinically meaningful improvement, as well as worsening, during construct validation is critical to providing practicing physicians, medical directors and health policy makers with a useful HRQL tool.
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Considering economic weights for traits during constructing selection indices can result in changing the magnitude of genetic correlation among these traits as a consequence of selection.
Examining the structural and biochemical changes during tissue construct growth within the self-assembling process sheds light on potential intervention strategies employing exogenous stimulation.
This 'relevancy' or 'importance' rating difference suggests that the cross-cultural performance of this item in particular should be subject to closer inspection during later construct validation activities.
This is a useful construct during tough economic times.
One reason that such effects are uncertain could be that what students construct during the tasks is largely unknown.
Analyses of the constructs of each of the 13 mathematics teachers revealed that 12 of the teachers (92%) elicited the CK construct during the RGT.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com