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Various compounds were added to the buffers for the duration of uptake (see figure legends for details).
Fig. 2C shows that when Caco-2 cell monolayers were incubated with PACAP (5 nM) in the basolateral solution for the duration of uptake, Gly Sar uptake was reduced.
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They simply remained in their home enclosure for the duration of the uptake period (40 minutes).
This procedure was repeated for the duration of the uptake period (40 minutes).
For both the COM and RES conditions, subjects were housed in their home enclosures for the duration of the uptake period.
For opsonised uptake the pneumococci were incubated in the presence pneumococcal antiserum for serotype 1 or 4 (Statens Serum Institut, Denmark) for the duration of the uptake period.
Depending on the severity and duration of iodide uptake reduction, perchlorate can reduce the amount of iodide stored in the thyroid (Brabant et al. 1992) and cause thyroid enlargement (U.S. EPA 2002) in humans.
The optimal concentration of leucine, and duration of leucine uptake, was determined by dose response and time course experiments, showing 1 mM l-leucine for 30 min to be optimal (SI Figures S5C and D).
The spatial localization of the alpha peaks was consistent with those observed during eye closure (Supporting information Fig. 1), which can be seen as an increase in the alpha amplitude above the baseline throughout the duration of drug uptake (Fig. 3C).
Therefore, investigations are required to identify most suitable species by their tolerance for arsenic, their duration of sustenance, arsenic uptake capacity, and simultaneously organic pollution reduction capacity.
Incubation of Caco-2 cell monolayers with the dietary phosphodiesterase inhibitor caffeine (5 mM) at both the apical and basolateral surfaces (for the duration of the 15 min uptake period) reduced Gly Sar uptake by around 50% (from 683.6 ± 18.9 to 351.1 ± 10.7 pmol cm− 2 [15 min]− 1, n = 17 18, p < 0.001, Fig. 1A).
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