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The doses used for irradiation, the duration of conditioning of culture medium on directly IR-exposed cells, and the length of bystander incubation were based on our previous studies and data from other groups published elsewhere [5], [19], [20], [21].
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Several durations of conditioning at 24 ± 3, 70 and 90 °C in distilled water were retained.
At shorter durations of conditioning at room temperature, a slight increase in strength and a slight decrease in modulus were observed; and at longer durations, 3000 h, a noticeable reduction in strength and strain-to-failure was observed.
It is not currently clear what the ideal human conditioning protocol is, in terms of duration, number of conditioning cycles and, importantly, whether this optimal protocol varies depending on which conditioning strategy is used.
The duration of the conditioning steps is an acceptable compromise between the necessity of applying long conditioning steps for achieving steady-state conditions and the practical need for mantaining the duration of each experiment within reasonable limits to avoid cell damage (Schönherr et al, 1999).
Conditioned suppression of drinking was assessed in response to presentation of the CS or an experimental background stimulus (flashing lights, previously presented for the duration of the conditioning session).
The long duration of the conditioning step was required by the slow kinetics of hERG1 channels (Schönherr et al, 1999) and is indifferent for IRK.
Along with the increase of the duration of the conditioning pulses, INa evoked by the test pulses decreased gradually, indicating a larger fraction of inactivated channels (Cha et al. 1999).
The fuel pellet is of annular design to enable operation at a peak linear power of 450 W/cm with the requirement of minimum duration of pre-conditioning.
The level of VEGF165 protein retained within depots (indicating general angiogenic factor production) was directly correlated to the duration of pre-conditioning.
For shorter durations of tensile conditioning (4 hours) and 8-minute heating, individual heating and cyclic tension did not produce a substantial effect, but combined stress treatments significantly altered HSP expression.
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