We derived five separate models, one for each of the following dependent variables: 1) contest outcome (won/lost) 2) aggression index; 3) number of bites; 4) duration of bites and 5) number of arm waves.
Contests were video-taped and lasted approximately 30 min. From the videos, we counted the number of approaches, number of chases, number of bites, duration of bites, number of arm waves (slow circumduction of left or right front limb) and number of times each lizard fled.
The duration of licking and biting of the injected paw was monitored over 0 5 min (early phase, neurogenic pain response) and 15 30 min (late phase, inflammatory pain response).
Cold allodynia was assessed by spraying acetone (100 μL) using an Eppendorf multistepper pipette onto the palmar surface of the ipsilateral hind paw through the wire mesh floor, and the duration of shaking, flinching, biting, or licking behavior that ensued in a 1 min period was measured [ 32, 33].
Number of cribbing bites, duration of cribbing behavior, and number of cribbing bouts were recorded during three 2-h observation sessions on d 20 of each period.
The parameters analyzed were age, sex, time of bite, time to take, duration of admission to resuscitation, clinical, therapeutic and severity of envenomation.
To determine if coral-associated gobies influence the duration of a single visit and/or the bite rate during these visits, median values were calculated for each trial.
We then recorded the number of bees biting and the duration of attack for each bee.
Although American- and southern-clade I. scapularis ticks are now considered 1 species, apparent differences exist in host-seeking behavior, biting behavior, and duration of attachment to different host types (9, 36, 37 ).
Figure 2 The distribution of open bite according to the duration of breastfeeding.
