Participants recruited for the dose-response study will be allocated after randomisation to consume either a placebo, 100 IU, 200 IU 400 IU, 800 IU or 1600 IU of alpha tocopherol orally each day for the duration determined by the time-course study.
For meiosis duration (determined with tubulin::GFP), timing was defined as follows: Meiosis I: time from entry into the spermatheca until tubulin::GFP waned to a small spot (the tubulin::GFP signal does not wane to undetectable before the meiosis II spindle waxes).
The search arrays were displayed for a certain duration (SOA) determined for each subject to obtain 75% correct performance, and then masked to reach a total trial duration of 500 ms. For this experiment the set size was fixed at 4 items, and only the presence or absence of the target varied randomly on each trial.
For palliative protocols where treatment duration was until disease progression, the actual number of cycles and/or months of treatment delivered to the patients recruited to each protocol was obtained from the patient records and an average treatment duration was determined for each protocol.
For adjuvant, neo-adjuvant and some palliative protocols which specified the treatment programme duration, costs were determined for the entire prespecified treatment programme.
An additional measure of performance stability in high performers were omissions in the 1.0-sec stimulus duration phase, as determined for 4 days after reaching criteria.
First, a duration score was determined for both tasks, reflecting the percentage of the overall time of spontaneous use of the affected arm and hand during either task (ranging from 0 to 100%).
Onset, offset, peak and duration of outbreaks were determined for each year.
Duration of hemodialysis was determined for each year by subtracting the earliest hemodialysis code from the last hemodialysis code within a given year.
The setup of experiments 1 and 2 entailed the presentation of stimuli for different durations that were individually determined for each CP participant and their respective matched controls.
Note that although this concentration and duration was optimal for targeting phenoloxidase in D. melanica, the optimal concentration and duration may have to be determined for specific species and clones of Daphnia as well as for different target genes using the RNAi via feeding system.
