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We confirmed the presence of this duplication using real time PCR to quantify genomic DNA template near the predicted ends of the duplication (Table S2) and subsequently used PCR with primers directed across the predicted duplication; such primers would only produce a product in the presence of a tandem duplication (reverse primers P3 or P4 and forward P11, see Figure 1 and Table S2).
We also tested for the relationship between gene expression and gene duplication, using gene family size as a proxy; and pathway position and gene duplication (using the terpenoid pathway as an example).
We also determined the mechanism of duplication using gene structure in the outgroup species (see supplementary methods, Supplementary Material online).
The relationship between alternative splicing and gene duplication using the newly updated data is worthy of further investigation.
Returned surveys were checked for duplication using three variables: age, sex, and geographical region where the CRC was working.
We make the first systematic attempt to use tree shape to study gene duplication using human gene phylogenies.
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Since EP duplication uses only one third the tape as standard-play (SP) duplication, manufacturing these videos costs much less.
Decreasing or increasing the rate of plasmid duplication used in the simulation shifted the center of the predicted distributions to the lower or higher values, respectively, than that using 84%, which does center on the average of the measured distributions.
Alignment was performed using STAR28, and de-duplication using Picard.
In this paper, we introduce and analyze two graph-based models for assigning orthologs in the presence of whole-genome duplications, using similarity information between pairs of genes.
We identified whole-genome duplications using an approach45,46 based on paralogue- and orthologue-specific comparisons of the 36,376 predicted protein-coding genes and further refined the estimated age of the lineage-specific WGD event using a phylogenetic dating approach13.
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