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Both TDD and TDDCI alleles contain similar duplication structures and should exhibit similar phenotypes.
This suggests that Bbp1 and possibly other pore membrane components of the SPB may localize to early SPB duplication structures.
Because cells released from the CDC20 arrest in a highly synchronized manner, we know we are examining the earliest duplication structures and comparing equivalent intermediates.
While we agree that the distance between spindle pole bodies is a useful method to determine the position the cell cycle, our study is the first to examine early SPB duplication structures using fluorescence microscopy, so what to use as a reference point to stage this period of pole duplication was unknown.
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This unique duplication structure suggests the two copies of the CSB2 and CSB3 may have arisen through occasional tandem duplication and subsequent concerted evolution.
Its complex rearrangement and duplication structure predispose it to non-allelic homologous recombinations that cause several micro-deletion disorders [5], [6].
We found that the Illumina Synthetic Long Reads assembly failed in the Mst77Y region, most likely because of its tandem duplication structure.
However, the duplication structure of the H2-Q region in C57BL/6 appears to be different to the mouse strain 129/SvJ [ 30].
In this respect, the duplicated XSR6 sequence will be a precious tool for analyzing polymorphism along this region among natural distorter chromosomes, with the goal of identifying a correlation between drive ability and duplication structure.
Our observation of an SPB duplication structure in mitosis is earlier than described by EM, perhaps because it is a transient step that is only observed in a small fraction of cells (Byers and Goetsch, 1974 , 1975.
Zody et al. carried out a detailed analysis of the duplication structure and history of chromosome 15 and reported that low copy repeats (LCRs), also termed segmental duplications (SDs), in chromosome 15 are largely clustered in proximal and distal 15q [ 40].
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