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The data was put through our sequencing pipeline consisting of base calling using the Illumina Pipeline, mapping and alignment with BWA [15], PCR duplication removal with SAMTools [16], and GATK [17] for variants calling.
One way to reduce amplification bias is duplication removal.
We used FASTX-Toolkit to perform trimming, quality filtering, and duplication removal [ 37].
The second update yielded 1727 records (1509 after duplication removal), which were assessed by titles and abstracts.
After duplication removal, 2909 rnpB sequences are unique, 2857 of which belong to bacA, 49 to arcA, 3 to bacB, but none for arcM, see Table 3.
The first update of the basic search resulted in 409 records, which were screened by title and abstract, and duplication removal resulted in 31 new full publications.
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Improvement of gene structure identification in three steps (detection of tandem duplications, removal of short introns, and exon intron boundary corrections) of our pipeline was validated (Supplementary Table S5).
Reads were cleaned using pyrocleaner [ 38], which applied a low-complexity filter followed by a read-size filter (over 100 bp) and a duplication-removal filter.
Duplicate removal.
Unlike for tandem duplications, the removal of dispersed duplications probably does not rely on homologous recombination between alleles, and thus their distribution may be less influenced by differences in the recombination environments of the neo-sex chromosomes.
Stasukevich goes on to explain that such elements as "crowd duplication, signage (addition and removal), and period-correct Manhattan skylines" were created digitally as well.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com