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The SSR, C-S/S ratio and catalyst concentration were investigated by a set of experiments that designed by "Design Expert" software in responsive surface central composite design (CCD) method with duplication of each test to improve confidence limits.
The second variant assumed much greater importance of the features resulting from localized points on code networks, depending on a five times duplication of each properly designated observation.
These results suggest that different mechanisms might be involved in the duplication of each intron type.
To distinguish these possibilities, the timing of duplication of each retained gene was pinpointed to an internal or external branch that led to C. reinhardtii.
The Plant Genome Duplication Database (PGDD) was utilized to analyze the duplication of each gene with a maximum distance of 500 kb permitted between collinear genes [ 100].
The PGD model required that after tandem duplication of each of the 13 genes, the 13 extra copies were moved to new locations and placed in the same order (and relative orientations) as their progenitors.
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To assess the reproducibility of the standard curve, standard reactions were performed three times independently including duplications of each reaction.
Segmental duplications of each WRKY gene within the family in soybean and Arabidopsis genomes were searched in the PGDD (http://chibba.agtec.uga.edu/duplication/).
However, for Psg30 and Psg31, independent duplications of each of the N2 and N3 domains were probably followed by a secondary duplication of the daughter domains, possibly as a single event.
Two main scenarios have been put forward to account for their presence: evolutionary remnants of chromosomal "en bloc" duplications or genome duplication, followed by gene loss and inversions [ 16, 17]; or they reflect independent tandem duplications of each gene family followed by adaptive groupings of genes on different chromosomes [ 18].
Production of the aircraft components would be split between Breguet and BAC and these would be assembled on two production lines; one in the UK and one in France, There would be no duplication of work; each component for the aircraft had only one source.
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