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We use duplication distance to measure the similarity between a pair of duplication blocks by counting the number of operations needed to generate Y from X in a simplest or most-parsimonious scenario.
To avoid duplication, we counted only follicles in which the nucleolus could be seen.
To quantify the length of the duplication, we counted the numbers of collinear cdBES in the duplicated SBs.
The duplication distance is the number of duplication events counted between the source and target genomes.
Among the eight low-tandem duplication families, none are in the low-segmental duplication category, and two of the eight have segmental duplication counts that place them approximately two standard deviations above the segmental-duplication median.
This is evident in total gene duplications that we counted (80673 in vertebrates vs. 33113 in pancrustaceans) but is not reflected in our other distance measures (denominators): both clades show similar genetic distance (as measured by average ortholog distance - 1047 and 814 respectively) as well as similar clade ages (as estimated by a molecular clock - 470 and 450 mya).
Duplication levels were assessed in raw reads by counting the occurrence of duplicates only in the starting 50bp of each read using the FastQC duplication level utility output, normalized to total metagenome size to reflect relative frequencies.
In this study, we evaluated gene duplicability by directly counting the number of gene duplication events using orthologous genes in closely related species.
Deletions and duplications were counted and analyzed separately.
If all duplicates in the local duplications of ohnologs are counted in ohnologs similar to a previous study (Bowers et al. 2003), the percentage of locally duplicated ohnologs increases to approximately 18%, suggesting that ohnologs and other types of genes may have comparable probabilities of being locally duplicated.
Total counts of duplications were also computed discarding the fraction of duplications that is expected to be more sensitive to error annotation: lineage-specific duplications with highly similar duplicates and duplications found in recently assembled genomes (see main text).
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