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We further used molecular, bioinformatics, and population-genetics approaches to define duplication contents, structural variants, and haplotypes within the common breakpoint.
Thus, genome-wide studies of segmental duplication contents become an effective measure to assess the quality of whole-genome sequence assemblies [ 36] and provide important information for the users of genome sequence.
To determine the duplication contents, we scanned every 500-bp window in the region by Blat (UCSC genome Browser) and plotted segments that have more than 90% sequence homology (> 100-bp) with other windows.
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First, duplication content varies significantly among different chromosomes.
In fact, the size and segmental duplication content of cold regions are correlated (r = 0.664).
We considered the genomic duplication content of the gene sets aligned to the bovine genome.
Human segmental duplication content of inversion breakpoints was determined using the whole genome assembly comparison approach (44).
First, searches of the trace server archives failed to find additional sequences whose high duplication content confounds assembly.
Most of this effect is due to an increase in intrachromosomal duplication content localized at specific clusters.
We present the first detailed genome-wide analysis of recent segmental duplication content of the bovine genome.
All of these factors must be taken into account during an assessment of recent segmental duplication content.
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